A 1-0 success for National League side Boreham Wood away at Championship high-flyers Bournemouth means they are the latest lower league team to make history with a stunning upset.
Mark Ricketts, a 37-year-old midfielder, proved to be the match-winner for the minnows as he struck an accurate effort from just outside the box.
The fifth-tier side showed plenty of resolve to earn their place in the last-16 and they have been rewarded with a trip to Goodison Park to face Everton.
The result certainly showed the magic of the FA Cup is still intact, despite the tournament generally being more and more devalued by the top clubs in the past decade.
Kidderminster Harriers were seconds away from shocking Europa League knockout stagers West Ham on Saturday, but were ultimately left with crushing disappointment.
Here are some more stunning moments from the world's most treasured knockout competition.
The name Ronnie Radford is synonymous with the FA Cup and it was his outstanding goal that saw Hereford take Newcastle to extra-time before Ricky George netted the winner.
It's easy to forget that this was actually a replay - Hereford managed to grab a 2-2 draw at St James' Park which was deemed a massive surprise in itself.
The then-Southern Football League outfit faced the Magpies for a second time and did brilliantly to keep them at bay before Malcolm Macdonald scored what looked like a winner in the 82nd minute.
Just three minutes later though, Radford - a part-time carpenter - fired home from 30 yards to send Edgar Street wild.
He then had a hand in George's winner which was quite easily Hereford's finest hour.
At the time, Sutton were plying their trade in the Conference, while Coventry were a staple of the English top-flight - the game looked destined to be an away banker.
However, the hosts took the lead three minutes before half-time when Tony Rains headed Mickey Stephens' corner past Steve Ogrizovic.
Coventry finally got into gear in the second half and got back on level terms when David Phillips found the net, but the winning goal came just prior to the hour mark when Matthew Hanlan scored a terrific volley.
No non-league team would beat a team from the top-flight for another 24 years when Luton Town triumphed at Norwich City and Sutton's manager Barrie Williams was thrilled at the manner of the win saying: "I am delighted that we won by playing good football."
This match between Division Four Wrexham and Division One Arsenal was a big shock with the Gunners the holders and an incredibly difficult team to beat under George Graham.
Paul Merson's first-half cross was headed home by Alan Smith and the Gunners held the lead until the 82nd minute.
However, the Welsh side rallied late on and goals from Mickey Thomas (not the Arsenal legend) and Steve Watkin turned the game around in sensational fashion.
Maybe this isn't a giant-killing in the conventional sense, but it was definitely the biggest final shock in recent memory.
City were the defending champions though Roberto Mancini's side failed to hold on to their title and the Italian was under pressure after spending big.
Wigan were battling relegation from the top-flight but to say the FA Cup gave them respite would be an understatement.
It was a final of few clear-cut opportunities, but Wigan took theirs deep into injury time when Shaun Maloney's corner was nodded in by Ben Watson and the travelling Latics fans became frenzied.
So many of their players shone on the day - the back four of Emmerson Boyce, Paul Scharner, Antolin Alcaraz, and Roger Espinosa were rock-solid, while man-of-the-match Callum McManaman tore City left-back Gael Clichy to shreds.
The win secured a first-ever major trophy for Wigan and certainly softened the blow of relegation which came just days later.
Meanwhile, Mancini lost his job at the end of that season and was replaced by Manuel Pellegrini.